The book provides latest information in a variety of subjects from pure micropropagation, conser-vation to transformation in multitude of crops. It will serve as a valuable reference book in most of the research laboratories. The chapters are derived from the Fourth International Conference held by Bangladesh Association for Plant Tissue Culture and Biotechnology.

In Vitro Culture: Somatic Embryogenesis in Stackhousia tryonii Bailey (Stackhousiaceae): A Rare and Nickel-hyperaccumulating Species of Central Queensland (Australia); In Vitro Regeneration and Hardening of Plantlets through Seed Culture in Spathoglottis plicata Blume; Effect of Cytokinins on Organogenesis and Callus Induction in Cotyledonary Explants of Tomato (Lycopersicon esculentum Mill.); Micropropagation and Conservation of the Threatened Medicinal Plants of Bangladesh: A Case Study of Alpinia calcarata Rosc.; Utilization of Plant Tissue Culture Technique for Mass Propagation and Conservation of Endan-gered Medicinal Plants; Variations in Betalain Contents among Different Cell Lines and some Factors Affecting the Biosynthesis of Betalain in Cell Culture of Portulaca sp. cv. 'Jewel'�Conservation: Low Temperature-induced G1-accumulated Cells in the Callus in Winter Wheat but not in Spring Wheat; Effect of Growth Regulators on Alnus nepalensis for Sustainable Agro-forestry in Nepal; A Novel Method of Germplasm Preservation of Trees through Excised Root Culture; How to Control Biodiversity by Means of Plant Biotechnology? Some Approaches to Overcoming Breeding Barriers; In Vitro Culture of YAM Seed (Dioscorea spp.); In Vitro Techniques to Improve the Germplasm Preservation-Case Studies for Three Temper-ate Crops and Some General Remarks�Transformation: Misidentification within Global Cacao Germplasm Collections; Molecular Markers-Strength, Limitations and Use in Plant Genetic Research; Breeding Rice for Salinity Tolerance using the Pokkali Allele: Finding a Linked DNA Marker�Molecular Markers: Lentil Improvement through Biotechnology; Bioengineered Rice for Improved Plant Protection and Nutritional Quality; Expression Stability and Gene Stacking of Recombinant Antifungal Genes in Transgenic Pea (Pisum sativum L.)